mLife已正式出版4期，报道内容涵盖微生物学领域的各个学科，下面10篇文章聚焦古菌研究，欢迎阅读！

Carl Woese: still ahead of our time 

Patrick Forterre

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该文介绍了Carl Woese的卓越学术贡献及其对作者本人的学术影响，包括Carl Woese将古细菌（Archaebacteria）改名为古菌（Archaea）。

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Forterre P. Carl Woese: still ahead of our time. mLife.  2022; 1: 359–367.

https://doi.org/10.1002/mlf2.12049

Inspiration and encounters: Carl Woese and my 30-year research journey

Yoshizumi Ishino

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该文介绍了Carl Woese的非凡学术成就，并回顾了作者本人在古菌及相关方面的研究历程。在作者的学术研究生涯中，Carl Woese一直激励和影响着他。

扫码阅读英文原文

Ishino Y. Inspiration and encounters: Carl Woese and my 30‐year research journey. mLife. 2022; 1: 368–373.

https://doi.org/10.1002/mlf2.12050

The expanding Asgard archaea and their elusive relationships with Eukarya 

Violette Da Cunha, Morgan Gaïa, Patrick Forterre

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The article implies that the Asgards were already diversified before the last eukaryotic common ancestor and shared the same biotopes with proto‐eukaryotes. The authors suggest that some Asgards might be still living in symbiosis today with modern Eukarya.

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Da Cunha V, Gaïa M, Forterre P. The expanding Asgard archaea and their elusive relationships with Eukarya. mLife. 2022; 1: 3–12.

https://doi.org/10.1002/mlf2.12012

The expanding Asgard archaea invoke novel insights into Tree of Life and eukaryogenesis

Zhichao Zhou, Yang Liu, Karthik Anantharaman, Meng Li

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该文聚焦于对Carl Woese生命之树的最新认识和发展。文章指出，地球生命之树是二域还是三域一直以来都是学术界的争论热点。近年来，阿斯加德古菌（Asgard Archaea）的发现和培养为这个争论提供了新的认知。

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Zhou Z, Liu Y, Anantharaman K, Li M. The expanding Asgard archaea invoke novel insights into Tree of Life and eukaryogenesis. mLife. 2022; 1: 374–381.

https://doi.org/10.1002/mlf2.12048

The archaeal KEOPS complex possesses a functional Gon7 homolog and has an essential function independent of the cellular t⁶A modification level

Pengju Wu, Qi Gan, Xuemei Zhang, Yunfeng Yang, Yuanxi Xiao, Qunxin She, Jinfeng Ni, Qihong Huang, Yulong Shen

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该研究团队系统研究了泉古菌冰岛硫化叶菌（Sulfolobus islandicus REY15A）KEOPS复合体的组成和功能，发现古菌编码的Pcc1-like蛋白是真核生物Gon7亚基的功能同源蛋白，同时发现KEOPS具有t⁶A修饰外的必需功能，推测为DNA同源重组修复。

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Wu P, Gan Q, Zhang X, Yang Y, Xiao Y, She Q, et al. The archaeal KEOPS complex possesses a functional Gon7 homolog and has an essential function independent of the cellular t⁶A modification level. mLife. 2023.

https://doi.org/10.1002/mlf2.12051

Reprogramming the endogenous type I CRISPR-Cas system for simultaneous gene regulation and editing in Haloarcula hispanica

Kaixin Du, Luyao Gong, Ming Li, Haiying Yu, Hua Xiang

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该研究团队对I型CRISPR-Cas系统功能进行了深入系统的研究，发现仅通过改造spacer长度，在不敲除cas3的情况下，利用I型系统可同时实现基因组编辑和基因转录调控。

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Du K, Gong L, Li M, Yu H, Xiang H. Reprogramming the endogenous type I CRISPR‐Cas system for simultaneous gene regulation and editing in Haloarcula hispanica. mLife. 2022; 1: 40–50.

https://doi.org/10.1002/mlf2.12010

Different outer membrane c-type cytochromes are involved in direct interspecies electron transfer to Geobacter or Methanosarcina species

Dawn E. Holmes, Jinjie Zhou, Jessica A. Smith, Caiqin Wang, Xinying Liu, Derek R. Lovley

This work demonstrates that the presence of outer-surface c‐type cytochromes does not necessarily confer the capacity for direct interspecies electron transfer (DIET) and emphasize the impact of the electron‐accepting partner on the physiology of the electron‐donating DIET partner.

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Holmes DE, Zhou J, Smith JA, Wang C, Liu X, Lovley DR. Different outer membrane c‐type cytochromes are involved in direct interspecies electron transfer to Geobacter or Methanosarcina species. mLife. 2022; 1: 272–286.

https://doi.org/10.1002/mlf2.12037

The unstable evolutionary position of Korarchaeota and its relationship with other TACK and Asgard archaea

Yang Liu, Meng Li

This work shows confident placements of (1) Korarchaeota as the basal branch to other TACK archaea and as a sister group to Asgard archaea; (2) Njordarchaeota at basal branch to Korarchaeota instead of within Asgard archaea.

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Liu Y, Li M. The unstable evolutionary position of Korarchaeota and its relationship with other TACK and Asgard archaea. mLife. 2022; 1: 218–222.

https://doi.org/10.1002/mlf2.12020

The late Archaean to early Proterozoic origin and evolution of anaerobic methane-oxidizing archaea

Yinzhao Wang, Ruize Xie, Jialin Hou, Zhenbo Lv, Liuyang Li, Yaoxun Hu, Hungchia Huang, Fengping Wang

This work suggests that the emergence of ANMEs may contribute to the reduction of methane in the late Archaean to early Proterozoic eon, which is an unappreciated potential cause that led to the Huronian Glaciation.

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Wang Y, Xie R, Hou J, Lv Z, Li L, Hu Y, Huang H, Wang F. The late Archaean to early Proterozoic origin and evolution of anaerobic methane‐oxidizing archaea. mLife. 2022; 1: 96–100.

https://doi.org/10.1002/mlf2.12013

Cytochrome-mediated direct electron uptake from metallic iron by Methanosarcina acetivorans

Dawn E. Holmes, Haiyan Tang, Trevor Woodard, Dandan Liang, Jinjie Zhou, Xinying Liu, Derek R. Lovley

This work suggests that the presence of multiheme c‐type cytochromes on corrosion surfaces might be diagnostic for direct metal‐to‐microbe electron transfer and that interfering with cytochrome function might be a strategy to mitigate corrosion.

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Holmes DE, Tang H, Woodard T, Liang D, Zhou J, Liu X, et al. Cytochrome‐mediated direct electron uptake from metallic iron by Methanosarcina  acetivorans. mLife.  2022; 1: 443–447.

https://doi.org/10.1002/mlf2.12044

——期刊新媒体部出品